purpose of protein ladder in western blot

#Purpose of protein ladder in western blot series

#Purpose of protein ladder in western blot series

#T7104A) is the most sensitive substrate in the series and is suitable for quantitative detection of very low-abundance proteins.

The Western BLoT Ultra Sensitive HRP Substrate (Cat.

Western BLoT Hyper substrate can also be used for X-ray film detection. Western BLoT Hyper is also appropriate in situations when the amount of primary antibody is limited and a high dilution factor is desired, or when the primary antibody has a relatively low binding affinity. #T7103A) is more sensitive than Western BLoT Quant and is suitable for quantitative detection of low-abundance proteins.

The Western BLoT Hyper HRP Substrate (Cat.

Western BLoT Quant substrate can also be used for X-ray film detection. In addition, this substrate produces a strong, long-lived signal, allowing long exposure times to enable the detection of low-abundance proteins. The signal from Western BLoT Quant is linear with respect to protein amount over a broad range of concentrations.

#T7102A) is intended for Western blot detection using a CCD camera. The Western BLoT Quant HRP Substrate (Cat.Although it is the most basic substrate in Western BLoT HRP substrate series, it produces a strong signal and provides high sensitivity with short exposure times. #T7101A) provides sensitivity suitable for detection using X-ray film. The Western BLoT Chemiluminescence HRP Substrate (Cat.Please refer to the table in the 'More Information' section below to compare the substrates' specifications. We offer four types of HRP substrates for Western blot detection. To use, equal volumes of the two solutions are mixed, the blot is incubated with the solution, and chemiluminescence is detected using film or digital imaging equipment. All of the substrates contain two components, a peroxide reagent and a luminol/enhancer solution. However this is typically a result of unspecific hybridisation.The Western BLoT HRP Substrate series are chemiluminescent substrates for detecting horseradish peroxidase (HRP)-labeled antibodies used during Western blotting. Due to high amount of the protein in each ladder band, the ladder might show positive results in immunodetection of blots.Use a bit less of the ladder (2-3 µl) for Western blots.Take 3-5 µl of the ladder for a small size gel slot for electrophoresis.Room-temperature-stable ladders, always ready to use.Monitoring of electrophoresis process and Western transfer efficiency.Approximate protein molecular weight determination on denaturing gels.– Research Fellow, Imperial College London We could use as little as 5µl which makes these markers cost effective.

The ladders also transfer well to membranes for use in Western Blotting and show up clearly in our Biorad imager. I have struggled to find markers which go over 250kDa of high quality and the CozyXL is by far the best one I have used so far. The ladders give nice distinct bands and stain very well using silver stain, not at all blurry and overstained which some of the ladders from market leaders do. We have recently bought and tested the CozyHi and CozyXL protein ladders for SDS-PAGE.